mouse monoclonal anti human pparγ2 (R&D Systems)
Structured Review

Mouse Monoclonal Anti Human Pparγ2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti human pparγ2/product/R&D Systems
Average 90 stars, based on 3 article reviews
Images
1) Product Images from "Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands "
Article Title: Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands
Journal: Molecular & Cellular Proteomics : MCP
doi: 10.1074/mcp.RA117.000308
Figure Legend Snippet: Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
Techniques Used: Control, SDS Page, Labeling, Transfection