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mouse monoclonal anti human pparγ2  (R&D Systems)


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    Structured Review

    R&D Systems mouse monoclonal anti human pparγ2
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Mouse Monoclonal Anti Human Pparγ2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti human pparγ2/product/R&D Systems
    Average 90 stars, based on 3 article reviews
    mouse monoclonal anti human pparγ2 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands "

    Article Title: Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1074/mcp.RA117.000308

    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
    Figure Legend Snippet: Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.

    Techniques Used: Control, SDS Page, Labeling, Transfection



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    R&D Systems mouse monoclonal anti human pparγ2
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
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    R&D Systems mouse monoclonal anti human ppar 2
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
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    R&D Systems mouse anti human ppar γ
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
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    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
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    R&D Systems mouse anti pparγ
    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged <t>PPARγ2</t> transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.
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    Image Search Results


    Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Unique Interactome Network Signatures for Peroxisome Proliferator-activated Receptor Gamma (PPARγ) Modulation by Functional Selective Ligands

    doi: 10.1074/mcp.RA117.000308

    Figure Lengend Snippet: Biotinylated DNA oligo capture and PPARγ IP in different cellular environments of ΔL1 cells. A, PPARγ IP in the cytoplasm and nucleus following 4-day ligand treatment using mouse monoclonal anti-human PPARγ antibody (m_hPPARγ). IgG control is shown in the middle. B, and C, 3× DR1 biotinylated oligo capture in the nucleus and cytoplasm following 1-day ligand treatment, respectively. D) PPARγ IP in the nucleus following 1-day treatment. The SDS-PAGE gels were transferred to nitrocellulose and proteins were visualized using rabbit polyclonal anti-human PPARγ antibody (r_hPPARγ), and a fluorescent labeled secondary antibody from rabbit to detect the respective IgG isotype. Band A indicates endogeneous PPARγ from ΔL1 cells where band B denotes human Flag-tagged PPARγ2 transfected in the HEK_293T cells. The arrow that is marked with a (?) is likely because of cross-reactivity with the antibody rather than PPARγ1 isoform. WBs were processed and analyzed using Licor Technology.

    Article Snippet: Primary mouse monoclonal anti-human PPARγ (sc-7273; 408–505; Lot#: L3013) for IP, rabbit polyclonal anti-human PPARγ (sc-7196; 8–108; Lot#: A2512) for Western blot (WB), and mouse IgG (sc-2025; Lot#: C1915) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) Mouse monoclonal anti-human PPARγ2 (PP-K8450B-00; 1–28; Lot#: A-2) for WB was obtained from R&D Systems (Minneapolis, MN).

    Techniques: Control, SDS Page, Labeling, Transfection